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Animals were aged and sex matched within individual experiments. Spots, each representing a single antibody-secreting cell, were counted using an ELIspot reader Autoimmune Diagnostika. These 12 cows were selected to represent the largest difference in gain during the ad libitum period. During the feed restriction, cows were weighed on days 0, 1, 14, 28, 56, 84, , and Proliferating B cells have the potential to differentiate into short-lived plasmablasts that provide the immediate, but low affinity, antibody that is important early in the immune response. Defective B cell receptor-mediated responses in mice lacking the Ets protein, Spi-B.
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